Costa Rica pulled off another 2014 World Cup upset by being Italy- officially knocking England out of the tournament.
England had a slim chance of squeezing through the group if they beat Costa Rica on Tuesday and other results went their way.
But a 44 minute Bryan Ruiz header was the only goal of the game tonight against Italy, eliminating England and setting up a three-horse race between themselves, Uruguay and Italy to qualify for the group.
Fans held their heads last night as England stood on the edge of World Cup elimination after suffering their second defeat in a row.
England battled hard and created clear goalscoring opportunities with Wayne Rooney hitting the crossbar in the first half.
But Luis Suarez gave Uruguay the lead from a Edinson Cavani cross after slack England defending.
Rooney then made up for his previous error by converting a Glen Johnson cross into an England equaliser.
But Liverpool striker Luis Suarez made sure that Uruguay had the last laugh, with an opportunist strike after more poor England defending.
Roy Hodgson was forced to deny that he was considering resigning after the match, which condemned England to their worst ever World Cup finals campaign.
At Teagues Bar last night the pre match optimism turned to frustration and despair at the end of the game, with many punters admitting that England's hopes of qualifying from the group stage are now extremely slim.
England appeared to have the heavens on their side in the second half as a white Porsche with the word 'Jesus' emblazened across the rear windscreen appeared in the pub driveway. England then promptly equalised.
However no sooner than the car had driven away, Uruguay took back the lead.
In Gloucester, no major trouble or disturbances were reported after England's disappointing result.
Gloucestershire police say there was one offence which was a domestic incident between a father and son in Tredworth, which they believe to be related to football.
Susan Tandy, landlady at Robert Raikkes House on Southgate Street, said: "We've never had a problem with football crowds in the six years since we've been here.
"I think there will still be a lot of interest in the World Cup and people visiting the pubs to watch the games, even if England are knocked out."
Michael and Marion Sage, pictured, landlord and landlady at The New Inn on Northgate Street, which showed the match on Thursday night, said: "There will still be interest in the World Cup even if England get knocked out. The semi finals and the final should get a lot of people coming in regardless.
"We're showing all the games and we've had no problems so far."
Around 3.5million football fans were expected to throw a 'sickie' for the game, which would have cost firms £350million.
Mark Owen, chairman of the South West England and Gloucestershire branch of the Federation of Small Businesses, pictured, said: "It's inexcusable for employees to pull sickies. It is very counter productive for small businesses."
Kieran O'Donoghue, from HR Champions, a personnel training and development company from Gloucester, said: "The most serious issue employers may face then is workers turning in to work whilst still under the influence of alcohol."
